Metabolic engineering of Deinococcus radiodurans for pinene
Por um escritor misterioso
Last updated 10 junho 2024
Background The objective of this work was to engineer Deinococcus radiodurans R1 as a microbial cell factory for the production of pinene, a monoterpene molecule prominently used for the production of fragrances, pharmaceutical products, and jet engine biofuels. Our objective was to produce pinene from glycerol, an abundant by-product of various industries. Results To enable pinene production in D. radiodurans, we expressed the pinene synthase from Abies grandis, the geranyl pyrophosphate (GPP) synthase from Escherichia coli, and overexpressed the native 1-deoxy-d-xylulose 5-phosphate synthase. Further, we disrupted the deinoxanthin pathway competing for the substrate GPP by either inactivating the gene dr0862, encoding phytoene synthase, or substituting the native GPP synthase with that of E. coli. These manipulations resulted in a D. radiodurans strain capable of producing 3.2 ± 0.2 mg/L pinene in a minimal medium supplemented with glycerol, with a yield of 0.13 ± 0.04 mg/g glycerol in shake flask cultures. Additionally, our results indicated a higher tolerance of D. radiodurans towards pinene as compared to E. coli. Conclusions In this study, we successfully engineered the extremophile bacterium D. radiodurans to produce pinene. This is the first study demonstrating the use of D. radiodurans as a cell factory for the production of terpenoid molecules. Besides, its high resistance to pinene makes D. radiodurans a suitable host for further engineering efforts to increase pinene titer as well as a candidate for the production of the other terpenoid molecules.
Biosynthesis, evolution and ecology of microbial terpenoids - Natural Product Reports (RSC Publishing) DOI:10.1039/D1NP00047K
Frontiers Lack of the Bacterial Phytochrome Protein Decreases Deinococcus radiodurans Resistance to Mitomycin C
Frontiers Proteomic and Metabolomic Profiling of Deinococcus radiodurans Recovering After Exposure to Simulated Low Earth Orbit Vacuum Conditions
Week 1 Discussion Micro.pdf - For your main discussion post first post you must choose from among the beneficial organisms below. Click Create Thread
Microbial synthesis of pinene.
PDF] Metabolic Engineering of Deinococcus radiodurans for the Production of Phytoene.
04 Terpenoides Compilation, PDF, Adenosine Diphosphate
Toward improved terpenoids biosynthesis: strategies to enhance the capabilities of cell factories, Bioresources and Bioprocessing
Pinene resistance of D. radiodurans ∆dr0862 P-I-D and E. coli EcpsB
Engineering Robustness of Microbial Cell Factories - Gong - 2017 - Biotechnology Journal - Wiley Online Library
Toward improved terpenoids biosynthesis: strategies to enhance the capabilities of cell factories, Bioresources and Bioprocessing
Engineering Robustness of Microbial Cell Factories - Gong - 2017 - Biotechnology Journal - Wiley Online Library
The amounts of pinene produced by different recombinant D. radiodurans
Proteometabolomic response of Deinococcus radiodurans exposed to UVC and vacuum conditions: Initial studies prior to the Tanpopo space mission
Deinococcus radiodurans - an overview
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