Advanced Quantitative Fluorescence Microscopy to Probe the Molecular Dynamics of Viral Entry, Science Lab

Viral entry into the host cell requires the coordination of many cellular and viral proteins in a precise order. Modern microscopy techniques are now allowing researchers to investigate these interactions with higher spatiotemporal resolution than ever before. Here we present two examples from the field of HIV research that make use of an innovative quantitative imaging approach as well as cutting edge fluorescence lifetime-based confocal microscopy methods to gain novel insights into how HIV fuses to cell membranes and enters the cell.

Detection of fluorescently-labelled influenza virus using

Molecular beacons. (a) The most common molecular beacon structure is a

Microscopy in Virology, Science Lab

2021 Top 10 Innovations The Scientist Magazine®

Chemosensors, Free Full-Text

Precision Fluorescent Labeling of an Adeno‐Associated Virus Vector to Monitor the Viral Infection Pathway - Zhang - 2018 - Biotechnology Journal - Wiley Online Library

HIV-1 binding and fusion events observed using confocal fluorescence

Probing the Allosteric Inhibition Mechanism of a Spike Protein Using Molecular Dynamics Simulations and Active Compound Identifications

Atomic force microscopy - Wikipedia

Measurement Assurance for Quantitative Cell Imaging by Optical Microscopy

Advanced Quantitative Fluorescence Microscopy to Probe the Molecular Dynamics of Viral Entry, Science Lab