The Chemokine CCL2 Increases Nav1.8 Sodium Channel Activity in Primary Sensory Neurons through a Gβγ-Dependent Mechanism
Por um escritor misterioso
Last updated 13 junho 2024
![The Chemokine CCL2 Increases Nav1.8 Sodium Channel Activity in Primary Sensory Neurons through a Gβγ-Dependent Mechanism](https://www.jneurosci.org/content/jneuro/31/50/18381/F1.large.jpg)
Changes in function of voltage-gated sodium channels in nociceptive primary sensory neurons participate in the development of peripheral hyperexcitability that occurs in neuropathic and inflammatory chronic pain conditions. Among them, the tetrodotoxin-resistant (TTX-R) sodium channel Nav1.8, primarily expressed by small- and medium-sized dorsal root ganglion (DRG) neurons, substantially contributes to the upstroke of action potential in these neurons. Compelling evidence also revealed that the chemokine CCL2 plays a critical role in chronic pain facilitation via its binding to CCR2 receptors. In this study, we therefore investigated the effects of CCL2 on the density and kinetic properties of TTX-R Nav1.8 currents in acutely small/medium dissociated lumbar DRG neurons from naive adult rats. Whole-cell patch-clamp recordings demonstrated that CCL2 concentration-dependently increased TTX-resistant Nav1.8 current densities in both small- and medium-diameter sensory neurons. Incubation with CCL2 also shifted the activation and steady-state inactivation curves of Nav1.8 in a hyperpolarizing direction in small sensory neurons. No change in the activation and inactivation kinetics was, however, observed in medium-sized nociceptive neurons. Our electrophysiological recordings also demonstrated that the selective CCR2 antagonist INCB3344 [ N -[2-[[(3 S ,4 S )-1- E 4-(1,3-benzodioxol-5-yl)-4-hydroxycyclohexyl]-4-ethoxy-3-pyrrolidinyl]amino]-2-oxoethyl]-3-(trifluoromethyl)benzamide] blocks the potentiation of Nav1.8 currents by CCL2 in a concentration-dependent manner. Furthermore, the enhancement in Nav1.8 currents was prevented by pretreatment with pertussis toxin (PTX) or gallein (a Gβγ inhibitor), indicating the involvement of Gβγ released from PTX-sensitive Gi/o-proteins in the cross talk between CCR2 and Nav1.8. Together, our data clearly demonstrate that CCL2 may excite primary sensory neurons by acting on the biophysical properties of Nav1.8 currents via a CCR2/Gβγ-dependent mechanism.
![The Chemokine CCL2 Increases Nav1.8 Sodium Channel Activity in Primary Sensory Neurons through a Gβγ-Dependent Mechanism](https://media.springernature.com/lw685/springer-static/image/art%3A10.1038%2Fs41598-017-07095-x/MediaObjects/41598_2017_7095_Fig14_HTML.jpg)
The analgesic activities of Stauntonia brachyanthera and YM11 through regulating inflammatory mediators and directly controlling the sodium channel prompt
![The Chemokine CCL2 Increases Nav1.8 Sodium Channel Activity in Primary Sensory Neurons through a Gβγ-Dependent Mechanism](https://ars.els-cdn.com/content/image/1-s2.0-S0014299923001577-gr4.jpg)
Targeting the chemokine ligand 2–chemokine receptor 2 axis provides the possibility of immunotherapy in chronic pain - ScienceDirect
![The Chemokine CCL2 Increases Nav1.8 Sodium Channel Activity in Primary Sensory Neurons through a Gβγ-Dependent Mechanism](https://www.jneurosci.org/content/jneuro/31/50/18381/F5.large.jpg)
The Chemokine CCL2 Increases Nav1.8 Sodium Channel Activity in Primary Sensory Neurons through a Gβγ-Dependent Mechanism
![The Chemokine CCL2 Increases Nav1.8 Sodium Channel Activity in Primary Sensory Neurons through a Gβγ-Dependent Mechanism](https://journals.sagepub.com/cms/10.1177/1744806916653969/asset/images/large/10.1177_1744806916653969-fig2.jpeg)
Functional inhibition of chemokine receptor CCR2 by dicer-substrate-siRNA prevents pain development - Valérie Bégin-Lavallée, Élora Midavaine, Marc-André Dansereau, Pascal Tétreault, Jean-Michel Longpré, Ashley M Jacobi, Scott D Rose, Mark A Behlke
![The Chemokine CCL2 Increases Nav1.8 Sodium Channel Activity in Primary Sensory Neurons through a Gβγ-Dependent Mechanism](https://www.dovepress.com/getfile_article_fulltext.php?filename=article_fulltext%2Fs415000%2F415127/img/DDDT_A_415127_O_F0004g.jpg)
Zhiqiao Gancao decoction on hyperalgesia in LDH
![The Chemokine CCL2 Increases Nav1.8 Sodium Channel Activity in Primary Sensory Neurons through a Gβγ-Dependent Mechanism](https://www.sciengine.com/figures/figures-98dbe217c9e74a72bdb6c8db23d80d6e-gmy123f01.png)
CC chemokine ligand 2 (CCL2) enhances TTX-sensitive sodium channel activity of primary afferent neurons in the complete Freud adjuvant-induced inflammatory pain model
![The Chemokine CCL2 Increases Nav1.8 Sodium Channel Activity in Primary Sensory Neurons through a Gβγ-Dependent Mechanism](https://ars.els-cdn.com/content/image/1-s2.0-S0014299923001577-gr1.jpg)
Targeting the chemokine ligand 2–chemokine receptor 2 axis provides the possibility of immunotherapy in chronic pain - ScienceDirect
![The Chemokine CCL2 Increases Nav1.8 Sodium Channel Activity in Primary Sensory Neurons through a Gβγ-Dependent Mechanism](https://journals.sagepub.com/cms/10.1177/1744806916653969/asset/images/large/10.1177_1744806916653969-fig6.jpeg)
Functional inhibition of chemokine receptor CCR2 by dicer-substrate-siRNA prevents pain development - Valérie Bégin-Lavallée, Élora Midavaine, Marc-André Dansereau, Pascal Tétreault, Jean-Michel Longpré, Ashley M Jacobi, Scott D Rose, Mark A Behlke
![The Chemokine CCL2 Increases Nav1.8 Sodium Channel Activity in Primary Sensory Neurons through a Gβγ-Dependent Mechanism](https://ars.els-cdn.com/content/image/1-s2.0-S0163725820301091-gr2.jpg)
Chemokines in chronic pain: cellular and molecular mechanisms and therapeutic potential - ScienceDirect
![The Chemokine CCL2 Increases Nav1.8 Sodium Channel Activity in Primary Sensory Neurons through a Gβγ-Dependent Mechanism](https://media.springernature.com/m685/springer-static/image/art%3A10.1186%2Fs13041-015-0105-2/MediaObjects/13041_2015_105_Fig4_HTML.gif)
Dexmedetomidine inhibits Tetrodotoxin-resistant Nav1.8 sodium channel activity through Gi/o-dependent pathway in rat dorsal root ganglion neurons, Molecular Brain
![The Chemokine CCL2 Increases Nav1.8 Sodium Channel Activity in Primary Sensory Neurons through a Gβγ-Dependent Mechanism](https://journals.sagepub.com/cms/10.1177/1744806916653969/asset/images/large/10.1177_1744806916653969-fig3.jpeg)
Functional inhibition of chemokine receptor CCR2 by dicer-substrate-siRNA prevents pain development - Valérie Bégin-Lavallée, Élora Midavaine, Marc-André Dansereau, Pascal Tétreault, Jean-Michel Longpré, Ashley M Jacobi, Scott D Rose, Mark A Behlke
![The Chemokine CCL2 Increases Nav1.8 Sodium Channel Activity in Primary Sensory Neurons through a Gβγ-Dependent Mechanism](https://www.jneurosci.org/content/jneuro/39/8/1539/F4.large.jpg)
Increased Resurgent Sodium Currents in Nav1.8 Contribute to Nociceptive Sensory Neuron Hyperexcitability Associated with Peripheral Neuropathies
![The Chemokine CCL2 Increases Nav1.8 Sodium Channel Activity in Primary Sensory Neurons through a Gβγ-Dependent Mechanism](https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fs41598-017-07095-x/MediaObjects/41598_2017_7095_Fig1_HTML.jpg)
The analgesic activities of Stauntonia brachyanthera and YM11 through regulating inflammatory mediators and directly controlling the sodium channel prompt
![The Chemokine CCL2 Increases Nav1.8 Sodium Channel Activity in Primary Sensory Neurons through a Gβγ-Dependent Mechanism](https://media.springernature.com/lw685/springer-static/image/art%3A10.1038%2Fs41598-017-07095-x/MediaObjects/41598_2017_7095_Fig11_HTML.jpg)
The analgesic activities of Stauntonia brachyanthera and YM11 through regulating inflammatory mediators and directly controlling the sodium channel prompt
![The Chemokine CCL2 Increases Nav1.8 Sodium Channel Activity in Primary Sensory Neurons through a Gβγ-Dependent Mechanism](https://www.jneurosci.org/content/jneuro/31/50/18381/F1.large.jpg)
The Chemokine CCL2 Increases Nav1.8 Sodium Channel Activity in Primary Sensory Neurons through a Gβγ-Dependent Mechanism
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