Turbo FISH: A Method for Rapid Single Molecule RNA FISH
Por um escritor misterioso
Last updated 11 junho 2024
Advances in RNA fluorescence in situ hybridization (RNA FISH) have allowed practitioners to detect individual RNA molecules in single cells via fluorescence microscopy, enabling highly accurate and sensitive quantification of gene expression. However, current methods typically employ hybridization times on the order of 2–16 hours, limiting its potential in applications like rapid diagnostics. We present here a set of conditions for RNA FISH (dubbed Turbo RNA FISH) that allow us to make accurate measurements with no more than 5 minutes of hybridization time and 3 minutes of washing, and show that hybridization times can go as low as 30 seconds while still producing quantifiable images. We further show that rapid hybridization is compatible with our recently developed iceFISH and SNP FISH variants of RNA FISH that enable chromosome and single base discrimination, respectively. Our method is simple and cost effective, and has the potential to dramatically increase the throughput and realm of applicability of RNA FISH.
PDF) Optimized protocol for single-molecule RNA FISH to visualize gene expression in S. cerevisiae
RNA Imaging with Multiplexed Error-Robust Fluorescence In Situ Hybridization (MERFISH).
Imaging of Telomerase RNA by Single-Molecule Inexpensive FISH Combined with Immunofluorescence - ScienceDirect
Strength in numbers: quantitative single‐molecule RNA detection assays - Gaspar - 2015 - WIREs Developmental Biology - Wiley Online Library
Paired Capture and FISH Detection of Individual Virions Enable Cell-Free Determination of Infectious Titers
Methods for spatial and temporal imaging of the different steps involved in RNA processing at single‐molecule resolution - Markey - 2021 - WIREs RNA - Wiley Online Library
Genome oligopaint via local denaturation fluorescence in situ hybridization - ScienceDirect
Strength in numbers: quantitative single‐molecule RNA detection assays - Gaspar - 2015 - WIREs Developmental Biology - Wiley Online Library
Single-molecule FISH in Drosophila muscle reveals location dependent mRNA composition of megaRNPs
Design, Labeling, and Application of Probes for RNA smFISH
Using Single Molecule mRNA Fluorescent in Situ Hybridization (RNA-FISH) to Quantify mRNAs in Individual Murine Oocytes and Embryos
IJMS, Free Full-Text
PTBP1 regulates injury responses and sensory pathways in adult peripheral neurons
Recomendado para você
-
Turbofish: Nomic Brings Bitcoin To The Cosmos!, by MVMT11 junho 2024
-
Easy Rust 047: Result part 3 (plus TURBOFISH)11 junho 2024
-
TurboFish: Любопытный и юркий Дип для игр в ванной!11 junho 2024
-
Solutions to the turbofish problem? : r/ProgrammingLanguages11 junho 2024
-
271 Turbo Fish Images, Stock Photos, 3D objects, & Vectors11 junho 2024
-
Coastal Pet Products Turbo Fish with Feathers - in Danbury, CT11 junho 2024
-
Gilt-head bream and Turbo fish11 junho 2024
-
Самоподсекающая удочка TurboFish 2,7 метра - Fire Tech11 junho 2024
-
The TurboFish Blog11 junho 2024
-
Idea] Turbofish replacement - language design - Rust Internals11 junho 2024
você pode gostar
-
QUAGMIRE E SUAS ORGIAS - UMA FAMÍLIA DA PESADA DUBLADO LINK PARA11 junho 2024
-
faz crossover entre New World e série A Roda do Tempo, do Prime Video11 junho 2024
-
Earned Glyph XP Does Not Roll Over in D4 - Use It or Lose It - Wowhead News11 junho 2024
-
2 more creatures confirmed for ark 2, stegosaurus and11 junho 2024
-
simon (tengen toppa gurren lagann) drawn by is_ii11 junho 2024
-
407 is now the second highest rated episode on imdb, still sad that its not at 10.0 anymore : r/MrRobot11 junho 2024
-
Akshon Esports on X: Did You Know: For the Japanese client, League of Legends' new champion Sett will be voiced by, Takehito Koyasu, the same VA for Dio Brando from JoJo's Bizarre11 junho 2024
-
Roms11 junho 2024
-
Bendy and the Ink Machine (Chapter 1+2+3+4) Free Download11 junho 2024
-
🌠AiNarata11 junho 2024