Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets, BMC Bioinformatics
Por um escritor misterioso
Last updated 13 junho 2024
![Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets, BMC Bioinformatics](https://media.springernature.com/m685/springer-static/image/art%3A10.1186%2Fs12859-022-05103-0/MediaObjects/12859_2022_5103_Fig4_HTML.png)
Background Long-read shotgun metagenomic sequencing is gaining in popularity and offers many advantages over short-read sequencing. The higher information content in long reads is useful for a variety of metagenomics analyses, including taxonomic classification and profiling. The development of long-read specific tools for taxonomic classification is accelerating, yet there is a lack of information regarding their relative performance. Here, we perform a critical benchmarking study using 11 methods, including five methods designed specifically for long reads. We applied these tools to several mock community datasets generated using Pacific Biosciences (PacBio) HiFi or Oxford Nanopore Technology sequencing, and evaluated their performance based on read utilization, detection metrics, and relative abundance estimates. Results Our results show that long-read classifiers generally performed best. Several short-read classification and profiling methods produced many false positives (particularly at lower abundances), required heavy filtering to achieve acceptable precision (at the cost of reduced recall), and produced inaccurate abundance estimates. By contrast, two long-read methods (BugSeq, MEGAN-LR & DIAMOND) and one generalized method (sourmash) displayed high precision and recall without any filtering required. Furthermore, in the PacBio HiFi datasets these methods detected all species down to the 0.1% abundance level with high precision. Some long-read methods, such as MetaMaps and MMseqs2, required moderate filtering to reduce false positives to resemble the precision and recall of the top-performing methods. We found read quality affected performance for methods relying on protein prediction or exact k-mer matching, and these methods performed better with PacBio HiFi datasets. We also found that long-read datasets with a large proportion of shorter reads (< 2 kb length) resulted in lower precision and worse abundance estimates, relative to length-filtered datasets. Finally, for classification methods, we found that the long-read datasets produced significantly better results than short-read datasets, demonstrating clear advantages for long-read metagenomic sequencing. Conclusions Our critical assessment of available methods provides best-practice recommendations for current research using long reads and establishes a baseline for future benchmarking studies.
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Frontiers SqueezeMeta, A Highly Portable, Fully Automatic
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Evaluation of taxonomic classification and profiling methods for
![Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets, BMC Bioinformatics](https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fs41467-022-30857-9/MediaObjects/41467_2022_30857_Fig1_HTML.png)
Short- and long-read metagenomics expand individualized structural
![Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets, BMC Bioinformatics](https://www.tandfonline.com/action/showGraphicalAbstractImage?doi=10.1080%2F10643389.2023.2181620&id=best_a_2181620_uf0001_c.jpg)
Full article: Recommendations for the use of metagenomics for
![Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets, BMC Bioinformatics](https://www.biorxiv.org/content/biorxiv/early/2022/02/02/2022.01.31.478527/F4.large.jpg)
Evaluation of taxonomic profiling methods for long-read shotgun
![Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets, BMC Bioinformatics](https://ars.els-cdn.com/content/image/1-s2.0-S0944501322000635-gr1.jpg)
Recovering metagenome-assembled genomes from shotgun metagenomic
![Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets, BMC Bioinformatics](https://media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40168-023-01557-3/MediaObjects/40168_2023_1557_Fig1_HTML.png)
Comparing genomes recovered from time-series metagenomes using
![Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets, BMC Bioinformatics](https://ars.els-cdn.com/content/image/3-s2.0-B9780124104723000051-f05-01-9780124104723.jpg)
Computational Tools for Taxonomic Microbiome Profiling of Shotgun
![Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets, BMC Bioinformatics](https://www.biorxiv.org/content/biorxiv/early/2022/11/15/2022.01.31.478527/F10.large.jpg)
Evaluation of taxonomic classification and profiling methods for
![Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets, BMC Bioinformatics](https://www.frontiersin.org/files/Articles/613791/fmicb-12-613791-HTML-r1/image_m/fmicb-12-613791-g001.jpg)
Frontiers Metagenomic Data Assembly – The Way of Decoding
![Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets, BMC Bioinformatics](https://journals.asm.org/cms/10.1128/msystems.00583-21/asset/18714c63-711f-470a-ade5-a3986c4c9aaf/assets/images/medium/msystems.00583-21-f001.gif)
METAnnotatorX2: a Comprehensive Tool for Deep and Shallow
![Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets, BMC Bioinformatics](https://media.springernature.com/m685/springer-static/image/art%3A10.1186%2Fs12859-022-05103-0/MediaObjects/12859_2022_5103_Fig2_HTML.png)
Evaluation of taxonomic classification and profiling methods for
![Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets, BMC Bioinformatics](https://www.researchgate.net/publication/364630785/figure/fig3/AS:11431281099357129@1669253905951/Percentage-of-reads-classified-correctly-or-incorrectly-in-Illumina-simulated-data-and.png)
Percentage of reads classified correctly or incorrectly in
Best-practice protocol for the acquisition and analysis of
![Evaluation of taxonomic classification and profiling methods for long-read shotgun metagenomic sequencing datasets, BMC Bioinformatics](https://www.frontiersin.org/files/Articles/814437/fgene-13-814437-HTML/image_m/fgene-13-814437-g001.jpg)
Frontiers MEDUSA: A Pipeline for Sensitive Taxonomic
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